Potential of the Liquid Fermentation of Fishery Waste by Paenibacillus elgii for Metalloprotease Production.

ABSTRACT: This study attempted to use fishery processing wastes to produce protease by Paenibacillus elgii TKU051. Of the tested wastes, tuna head powder (THP) was found to be the most effective carbon and nitrogen (C/N) source, and the optimal conditions were as follows: 0.811% THP, 0.052% K₂HPO₄, 0.073% MgSO₄, initial pH of 8.96, incubation temperature of 31.4 °C, and incubation time of 3.092 days to achieve the maximum protease activity of 2.635 ± 0.124 U/mL. A protease with a molecular weight of 29 kDa was purified and biochemically characterized. Liquid chromatography with tandem mass spectrometry analysis revealed an amino acid sequence of STVHYSTR of P. elgii TKU051 protease, suggesting that the enzyme may belong to the M4 family of metalloprotea

SUBMITTER: Doan CT 

PROVIDER: S-EPMC9268979 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature