Complete Genome Sequence of a Novel Monopartite <i>Mastrevirus</i>, Soybean Geminivirus B, Isolated from Soybean (<i>Glycine max</i> (L.) Merrill).

ABSTRACT: Soybean is one of the most important crops in Korea. To identify the viruses infecting soybean, we conducted RNA sequencing with samples displaying symptoms of viral disease. A contig displaying sequence similarity to the known Geminivirus was identified. A polymerase chain reaction (PCR) using two different pairs of back-to-back primers and rolling circle amplification (RCA) confirmed the complete genome of a novel virus named soybean geminivirus B (SGVB), consisting of a circular monopartite DNA genome measuring 2616 nucleotides (nt) in length. SGVB contains four open reading frames (ORFs) and three intergenic regions (IRs). IR1 includes a nonanucleotide origin of replication in the stem-loop structure. Phylogenetic and BLAST analyses demonstrated that SGVB could be a novel virus

SUBMITTER: Choi H 

PROVIDER: S-EPMC9269612 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature