Hydrogel-based biocontainment of bacteria for continuous sensing and computation.

ABSTRACT: Genetically modified microorganisms (GMMs) can enable a wide range of important applications including environmental sensing and responsive engineered living materials. However, containment of GMMs to prevent environmental escape and satisfy regulatory requirements is a bottleneck for real-world use. While current biochemical strategies restrict unwanted growth of GMMs in the environment, there is a need for deployable physical containment technologies to achieve redundant, multi-layered and robust containment. We developed a hydrogel-based encapsulation system that incorporates a biocompatible multilayer tough shell and an alginate-based core. This deployable physical containment strategy (DEPCOS) allows no detectable GMM escape, bacteria to be protected against environmental insults incl

SUBMITTER: Tang TC 

PROVIDER: S-EPMC9269716 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature