Intact Drosophila central nervous system cellular quantitation reveals sexual dimorphism.

ABSTRACT: Establishing with precision the quantity and identity of the cell types of the brain is a prerequisite for a detailed compendium of gene and protein expression in the central nervous system (CNS). Currently, however, strict quantitation of cell numbers has been achieved only for the nervous system of Caenorhabditis elegans. Here, we describe the development of a synergistic pipeline of molecular genetic, imaging, and computational technologies designed to allow high-throughput, precise quantitation with cellular resolution of reporters of gene expression in intact whole tissues with complex cellular constitutions such as the brain. We have deployed the approach to determine with exactitude the number of functional neurons and glia in the entire intact larval Drosophila CNS, r

SUBMITTER: Jiao W 

PROVIDER: S-EPMC9270032 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature