GLI3 regulates muscle stem cell entry into G_Alert and self-renewal.

ABSTRACT: Satellite cells are required for the growth, maintenance, and regeneration of skeletal muscle. Quiescent satellite cells possess a primary cilium, a structure that regulates the processing of the GLI family of transcription factors. Here we find that GLI3 processing by the primary cilium plays a critical role for satellite cell function. GLI3 is required to maintain satellite cells in a G₀ dormant state. Strikingly, satellite cells lacking GLI3 enter the G_Alert state in the absence of injury. Furthermore, GLI3 depletion stimulates expansion of the stem cell pool. As a result, satellite cells lacking GLI3 display rapid cell-cycle entry, increased proliferation and augmented self-renewal, and markedly enhanced regenerative capacity. At the molecular level, we establish

SUBMITTER: Brun CE 

PROVIDER: S-EPMC9270324 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature