Performance and reproducibility of ¹³C and ¹⁵N hyperpolarization using a cryogen-free DNP polarizer.

ABSTRACT: The setup, operational procedures and performance of a cryogen-free device for producing hyperpolarized contrast agents using dissolution dynamic nuclear polarization (dDNP) in a preclinical imaging center is described. The polarization was optimized using the solid-state, DNP-enhanced NMR signal to calibrate the sample position, microwave and NMR frequency and power and flip angle. The polarization of a standard formulation to yield ~ 4 mL, 60 mM 1-¹³C-pyruvic acid in an aqueous solution was quantified in five experiments to P(¹³C) = (38 ± 6) % (19 ± 1) s after dissolution. The mono-exponential time constant of the build-up of the solid-state polarization was quantified to (1032 ± 22) s. We achieved a duty cycle of 1.5 h that includes sample loading, monitoring the p

SUBMITTER: Ferrari A 

PROVIDER: S-EPMC9270333 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature