ScPCOR-seq enables co-profiling of chromatin occupancy and RNAs in single cells.

ABSTRACT: Cell-to-cell variation in gene expression is a widespread phenomenon, which may play important roles in cellular differentiation, function, and disease development^1-9. Chromatin is implicated in contributing to the cellular heterogeneity in gene expression^10-16. Fully understanding the mechanisms of cellular heterogeneity requires simultaneous measurement of RNA and occupancy of histone modifications and transcription factors on chromatin due to their critical roles in transcriptional regulation^17,18. We generally term the occupancy of histone modifications and transcription factors as Chromatin occupancy. Here, we report a technique, termed scPCOR-seq (single-cell Profiling of Chromatin Occupancy and RNAs Sequencing), for simultaneously profiling genome-wi

SUBMITTER: Pan L 

PROVIDER: S-EPMC9270334 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature