Multi-omic characterization of pediatric ARDS via nasal brushings.

ABSTRACT:

Rationale

While nasal brushing transcriptomics can identify disease subtypes in chronic pulmonary diseases, it is unknown whether this is true in pediatric acute respiratory distress syndrome (PARDS).

Objectives

Determine whether nasal transcriptomics and methylomics can identify clinically meaningful PARDS subgroups that reflect important pathobiological processes.

Methods

Nasal brushings and serum were collected on days 1, 3, 7, and 14 from control and PARDS subjects from two centers. PARDS duration was the primary endpoint.

Measurements and main results

Twenty-four control and 39 PARDS subjects were enrolled. Two nasal methylation patterns were identified. Compared to Methyl Subgroup 1, Subgroup 2 had hypomethylation of inflammatory genes and was enriched fo

SUBMITTER: Williams JG 

PROVIDER: S-EPMC9270778 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature