Uncoupling transcription and translation through miRNA-dependent poly(A) length control in haploid male germ cells.

ABSTRACT: As one of the post-transcriptional regulatory mechanisms, uncoupling of transcription and translation plays an essential role in development and adulthood physiology. However, it remains elusive how thousands of mRNAs get translationally silenced while stability is maintained for hours or even days before translation. In addition to oocytes and neurons, developing spermatids display significant uncoupling of transcription and translation for delayed translation. Therefore, spermiogenesis represents an excellent in vivo model for investigating the mechanism underlying uncoupled transcription and translation. Through full-length poly(A) deep sequencing, we discovered dynamic changes in poly(A) length through deadenylation and re-polyadenylation. Deadenylation appeared to be mediated by micro

SUBMITTER: Guo M 

PROVIDER: S-EPMC9270972 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature