Interchromosomal interaction of homologous Stat92E alleles regulates transcriptional switch during stem-cell differentiation.

ABSTRACT: Pairing of homologous chromosomes in somatic cells provides the opportunity of interchromosomal interaction between homologous gene regions. In the Drosophila male germline, the Stat92E gene is highly expressed in a germline stem cell (GSC) and gradually downregulated during the differentiation. Here we show that the pairing of Stat92E is always tight in GSCs and immediately loosened in differentiating daughter cells, gonialblasts (GBs). Disturbance of Stat92E pairing by relocation of one locus to another chromosome or by knockdown of global pairing/anti-pairing factors both result in a failure of Stat92E downregulation, suggesting that the pairing is required for the decline in transcription. Furthermore, the Stat92E enhancer, but not its transcription, is required for the change in pairi

SUBMITTER: Antel M 

PROVIDER: S-EPMC9271046 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature