Controlling Morphology and Functions of Cardiac Organoids by Two-Dimensional Geometrical Templates.

ABSTRACT: Traditionally, tissue-specific organoids are generated as 3D aggregates of stem cells embedded in Matrigel or hydrogels, and the aggregates eventually end up a spherical shape and suspended in the matrix. Lack of geometrical control of organoid formation makes these spherical organoids limited for modeling the tissues with complex shapes. To address this challenge, we developed a new method to generate 3D spatial-organized cardiac organoids from 2D micropatterned human induced pluripotent stem cell (hiPSC) colonies, instead of directly from 3D stem cell aggregates. This new approach opens the possibility to create cardiac organoids that are templated by 2D non-spherical geometries, which potentially provides us a deeper understanding of biophysical controls on developmental organogenesis.

SUBMITTER: Hoang P 

PROVIDER: S-EPMC9271134 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature