Topoisomerase VI participates in an insulator-like function that prevents H3K9me2 spreading.

ABSTRACT: The organization of the genome into transcriptionally active and inactive chromatin domains requires well-delineated chromatin boundaries and insulator functions in order to maintain the identity of adjacent genomic loci with antagonistic chromatin marks and functionality. In plants that lack known chromatin insulators, the mechanisms that prevent heterochromatin spreading into euchromatin remain to be identified. Here, we show that DNA Topoisomerase VI participates in a chromatin boundary function that safeguards the expression of genes in euchromatin islands within silenced heterochromatin regions. While some transposable elements are reactivated in mutants of the Topoisomerase VI complex, genes insulated in euchromatin islands within heterochromatic regions of the Arabidopsis thalian

SUBMITTER: Meteignier LV 

PROVIDER: S-EPMC9271158 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature