Localization of a TORC1-eIF4F translation complex during CD8⁺ T cell activation drives divergent cell fate.

ABSTRACT: Activated CD8⁺ T lymphocytes differentiate into heterogeneous subsets. Using super-resolution imaging, we found that prior to the first division, dynein-dependent vesicular transport polarized active TORC1 toward the microtubule-organizing center (MTOC) at the proximal pole. This active TORC1 was physically associated with active eIF4F, required for the translation of c-myc mRNA. As a consequence, c-myc-translating polysomes polarized toward the cellular pole proximal to the immune synapse, resulting in localized c-myc translation. Upon division, the TORC1-eIF4A complex preferentially sorted to the proximal daughter cell, facilitating asymmetric c-Myc synthesis. Transient disruption of eIF4A activity at first division skewed long-term cell fate trajectories to memory-like functi

SUBMITTER: Liedmann S 

PROVIDER: S-EPMC9271638 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature