Development and Application of Transcription Terminators for Polyhydroxylkanoates Production in Halophilic Halomonas bluephagenesis TD01.

ABSTRACT: Halomonas bluephagenesis TD01 is one of the ideal chassis for low-cost industrial production based on "Next Generation Industrial Biotechnology," yet the limited genetically regulatory parts such as transcriptional terminators, which are crucial for tuned regulations on gene expression, have hampered the engineering and applications of the strain. In this study, a series of intrinsic Rho-independent terminators were developed by either genome mining or rational design, and seven of them proved to exhibit higher efficiencies than the canonical strong T7 terminator, among which three terminators displayed high efficiencies over 90%. A preliminary modeling on the sequence-efficiency relationship of the terminators suggested that the poly U sequence regularity, the length and GC content

SUBMITTER: Xu M 

PROVIDER: S-EPMC9271916 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature