Modification of Fibronectin by Non-Enzymatic Glycation Impairs K⁺ Channel Function in Rat Cerebral Artery Smooth Muscle Cells.

ABSTRACT: Fibronectin (FN) enhances K⁺ channel activity by integrin-mediated mechanisms. As vascular smooth muscle (VSM) K⁺ channels mediate vasodilation, we hypothesized that modification of fibronectin, via advanced non-enzymatic glycation, would alter signaling of this extracellular matrix protein through these channels. Bovine FN (1 mg/ml) was glycated (gFN) for 5 days using methylglyoxal (50 mM), and albumin was similarly glycated as a non-matrix protein control. VSM cells were isolated from rat cerebral arteries for measurement of macroscopic K⁺ channel activity using whole cell patch clamp methodology. Pharmacological inhibitors, iberiotoxin (0.1 μM) and 4-aminopyridine (0.1 mM), were used to identify contributions of large-conductance, Ca²⁺-activat

SUBMITTER: Yang Y 

PROVIDER: S-EPMC9272009 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature