METTL14-mediated epitranscriptome modification of MN1 mRNA promote tumorigenicity and all-trans-retinoic acid resistance in osteosarcoma.

ABSTRACT:

Background

Osteosarcoma (OS) is the most common primary malignant bone tumor in adolescents. The molecular mechanism behind OS progression and metastasis remains poorly understood, which limits the effectiveness of current therapies. RNA N⁶-methyladenosine (m⁶A) modification plays a critical role in influencing RNA fate. However, the biological significance of m⁶A modification and its potential regulatory mechanisms in the development of OS remain unclear.

Methods

Liquid chromatography-tandem mass spectrometry (LC-MS/MS), dot blotting, and colorimetric ELISA were used to detect m⁶A levels. Western blotting, quantitative real-time PCR (RT-qPCR) and immunohistochemistry (IHC) were used to investigate METTL14 expression levels. Methyl

SUBMITTER: Li HB 

PROVIDER: S-EPMC9272358 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature