Recognition of 3' nucleotide context and stop codon readthrough are determined during mRNA translation elongation.

ABSTRACT: The nucleotide context surrounding stop codons significantly affects the efficiency of translation termination. In eukaryotes, various 3' contexts that are unfavorable for translation termination have been described; however, the exact molecular mechanism that mediates their effects remains unknown. In this study, we used a reconstituted mammalian translation system to examine the efficiency of stop codons in different contexts, including several previously described weak 3' stop codon contexts. We developed an approach to estimate the level of stop codon readthrough in the absence of eukaryotic release factors (eRFs). In this system, the stop codon is recognized by the suppressor or near-cognate tRNAs. We observed that in the absence of eRFs, readthrough occurs in a 3' nucleotide context-

SUBMITTER: Biziaev N 

PROVIDER: S-EPMC9272376 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature