Mitochondrial morphology regulates organellar Ca²⁺ uptake and changes cellular Ca²⁺ homeostasis.

ABSTRACT: Changes in mitochondrial size and shape have been implicated in several physiologic processes, but their role in mitochondrial Ca²⁺ uptake regulation and overall cellular Ca²⁺ homeostasis is largely unknown. Here we show that modulating mitochondrial dynamics toward increased fusion through expression of a dominant negative (DN) form of the fission protein [dynamin-related protein 1 (DRP1)] markedly increased both mitochondrial Ca²⁺ retention capacity and Ca²⁺ uptake rates in permeabilized C2C12 cells. Similar results were seen using the pharmacological fusion-promoting M1 molecule. Conversely, promoting a fission phenotype through the knockdown of the fusion protein mitofusin (MFN)-2 strongly reduced the mitochondrial Ca²⁺ uptake spee

SUBMITTER: Kowaltowski AJ 

PROVIDER: S-EPMC9272750 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature