Multi-locus evaluation of gastrointestinal bacterial communities from Zalophus californianus pups in the Gulf of California, Mexico.

ABSTRACT:

Background

The gastrointestinal (GI) bacterial communities of sea lions described to date have occasionally revealed large intraspecific variability, which may originate from several factors including different methodological approaches. Indeed, GI bacterial community surveys commonly rely on the use of a single hypervariable region (HR) of 16S rRNA, which may result in misleading structural interpretations and limit comparisons among studies. Here, we considered a multi-locus analysis by targeting six HRs of 16S rRNA with the aims of (i) comprehensively assessing the GI bacterial consortium in rectal samples from Zalophus californianus pups and (ii) elucidating structural variations among the tested HRs. In addition, we evaluated which HRs may be most suitable

SUBMITTER: Ramirez-Delgado D 

PROVIDER: S-EPMC9272818 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature