Preferential Regulation of Transient Protein-Protein Interaction by the Macromolecular Crowders.

ABSTRACT: The environmental condition is a critical regulation factor for protein behavior in solution. Several studies have shown that macromolecular crowders can modulate protein structures, interactions, and functions. Recent publications described the regulation of specific interaction by macromolecular crowders. However, the other category of protein-protein interaction, namely, the transient interaction, is rarely investigated, especially from the perspective of protein structure to study transient interactions between proteins. Here, we used nuclear magnetic resonance and small-angle X-ray/neutron scattering methods to structurally investigate the ensemble of the protein complex in dilute buffer and crowded environments. Histidine phosphocarrier protein (HPr) and the N-terminal domain of enzy

SUBMITTER: Gong Z 

PROVIDER: S-EPMC9272825 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature