Ct values as a diagnostic tool for monitoring SARS-CoV-2 viral load using the QIAstat-Dx® Respiratory SARS-CoV-2 Panel.

ABSTRACT:

Objectives

Qualitative real-time polymerase chain reaction tests are not designed to provide quantitative or semiquantitative results because cycle threshold (Ct) values are not normalized to standardized controls of known concentration. The aim of this study was to characterize SARS-CoV-2 viral loads based on Ct values, using the QIAstat-Dx® Respiratory SARS-CoV-2 Panel.

Methods

Different lineages of SARS-CoV-2 clinical samples and the World Health Organization international standard were used to assess the linearity of the QIAstat-Dx Respiratory SARS-CoV-2 Panel. Limit of detection for the different lineages was characterized.

Results

Comparable efficiencies and linearity for all samples resulted in R² ≥0.99, covering a dynamic range of 1,000,000-100 copi

SUBMITTER: Juanola-Falgarona M 

PROVIDER: S-EPMC9273520 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature