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Regulation of AUXIN RESPONSE FACTOR condensation and nucleo-cytoplasmic partitioning.

ABSTRACT: Auxin critically regulates plant growth and development. Auxin-driven transcriptional responses are mediated through the AUXIN RESPONSE FACTOR (ARF) family of transcription factors. ARF protein condensation attenuates ARF activity, resulting in dramatic shifts in the auxin transcriptional landscape. Here, we perform a forward genetics screen for ARF hypercondensation, identifying an F-box protein, which we named AUXIN RESPONSE FACTOR F-BOX1 (AFF1). Functional characterization of SCF^AFF1 revealed that this E3 ubiquitin ligase directly interacts with ARF19 and ARF7 to regulate their accumulation, condensation, and nucleo-cytoplasmic partitioning. Mutants defective in AFF1 display attenuated auxin responsiveness, and developmental defects, suggesting that SCF^AFF1 -mediated regulation of ARF protein drives aspects of auxin response and plant development.

SUBMITTER: Jing H

PROVIDER: S-EPMC9273615 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature

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Regulation of AUXIN RESPONSE FACTOR condensation and nucleo-cytoplasmic partitioning.

Jing Hongwei H Korasick David A DA Emenecker Ryan J RJ Morffy Nicholas N Wilkinson Edward G EG Powers Samantha K SK Strader Lucia C LC

Nature communications 20220711 1

Auxin critically regulates plant growth and development. Auxin-driven transcriptional responses are mediated through the AUXIN RESPONSE FACTOR (ARF) family of transcription factors. ARF protein condensation attenuates ARF activity, resulting in dramatic shifts in the auxin transcriptional landscape. Here, we perform a forward genetics screen for ARF hypercondensation, identifying an F-box protein, which we named AUXIN RESPONSE FACTOR F-BOX1 (AFF1). Functional characterization of SCF<sup>AFF1</su ...[more]

PMID: 35817767

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