Modulation of Gap Junction Coupling Within the Islet of Langerhans During the Development of Type 1 Diabetes.

ABSTRACT: In type 1 diabetes (T1D), islet dysfunction occurs prior to diabetes onset. Pro-inflammatory cytokines can disrupt insulin secretion and Ca²⁺ homeostasis. Connexin36 (Cx36) gap junctions electrically couple β-cells to coordinate glucose-stimulated Ca²⁺ and insulin secretion. Cx36 gap junction coupling can also protect against cytokine-induced apoptosis. Our goal was to determine how islet gap junction coupling and Ca²⁺ dynamics are altered in mouse models of T1D prior to diabetes. Glucose tolerance was assessed in NOD and immunodeficient NOD-RAG1KO mice at 6-12 weeks age. Glucose-stimulated insulin secretion, Ca²⁺ dynamics, and gap junction coupling were measured in islets isolated at each age. Gap junction coupling was also measured in islets fr

SUBMITTER: Farnsworth NL 

PROVIDER: S-EPMC9274093 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature