CD166 Engagement Augments Mouse and Human Hematopoietic Progenitor Function via Activation of Stemness and Cell Cycle Pathways.

ABSTRACT: Hematopoietic stem (HSC) and progenitor (HPC) cells are regulated by interacting signals and cellular and noncellular elements of the hematopoietic niche. We previously showed that CD166 is a functional marker of murine and human HSC and of cellular components of the murine niche. Selection of murine CD166⁺ engrafting HSC enriched for marrow repopulating cells. Here, we demonstrate that CD166-CD166 homophilic interactions enhance generation of murine and human HPC in vitro and augment hematopoietic function of these cells. Interactions between cultured CD166⁺ Lineage^- Sca-1⁺ c-Kit⁺ (LSK) cells and CD166⁺ osteoblasts (OBs) significantly enhanced the expansion of colony-forming units (CFUs). Interactions between CD166⁺

SUBMITTER: Zhang J 

PROVIDER: S-EPMC9274619 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature