Targeting breast cancer with a combination of DNT and LAG3 checkpoint blockage and its mechanism.

ABSTRACT:

Introduction

The characteristics of the tumor immune microenvironment (TIME) are closely related to immunotherapy. Breast cancer can benefit from immunotherapy, and its TIME is still unclear.

Methods

We utilized mass cytometry to explore the immune cell heterogeneity in breast cancer. Double-negative T cells (DNTs) from healthy volunteers (HBs) were enriched in vitro. Flow cytometry was used to detect the cell surface receptors of cancer cells and DNT cells. The correlation between immune checkpoints and the abundance of immune cells or prognosis of breast cancer was analyzed by the TCGA database. The messenger RNA (mRNA) expression of functional genes was performed by quantitative real-time PCR.

Results

We found that the frequencies of Granzyme B (GZMB)⁺ C

SUBMITTER: Wang M 

PROVIDER: S-EPMC9274802 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature