Ontology highlight
ABSTRACT:
SUBMITTER: Emert-Sedlak LA
PROVIDER: S-EPMC9274903 | biostudies-literature | 2022 Jan
REPOSITORIES: biostudies-literature
Emert-Sedlak Lori A LA Moukha-Chafiq Omar O Shi Haibin H Du Shoucheng S Alvarado John J JJ Pathak Vibha V Tanner Samuel G SG Hunter Robert N RN Nebane Miranda M Chen Li L Ilina Tatiana V TV Ishima Rieko R Zhang Sixue S Kuzmichev Yury V YV Wonderlich Elizabeth R ER Schader Susan M SM Augelli-Szafran Corinne E CE Ptak Roger G RG Smithgall Thomas E TE
ACS infectious diseases 20220105 1
HIV-1 Nef is an attractive target for antiretroviral drug discovery because of its role in promoting HIV-1 infectivity, replication, and host immune system avoidance. Here, we applied a screening strategy in which recombinant HIV-1 Nef protein was coupled to activation of the Src-family tyrosine kinase Hck, which enhances the HIV-1 life cycle in macrophages. Nef stimulates recombinant Hck activity <i>in vitro</i>, providing a robust assay for chemical library screening. High-throughput screening ...[more]