Metagenomic prediction of antimicrobial resistance in critically ill patients with lower respiratory tract infections.

ABSTRACT:

Background

Antimicrobial resistance (AMR) is rising at an alarming rate and complicating the management of infectious diseases including lower respiratory tract infections (LRTI). Metagenomic next-generation sequencing (mNGS) is a recently established method for culture-independent LRTI diagnosis, but its utility for predicting AMR has remained unclear. We aimed to assess the performance of mNGS for AMR prediction in bacterial LRTI and demonstrate proof of concept for epidemiological AMR surveillance and rapid AMR gene detection using Cas9 enrichment and nanopore sequencing.

Methods

We studied 88 patients with acute respiratory failure between 07/2013 and 9/2018, enrolled through a previous observational study of LRTI. Inclusion criteria were age ≥ 18, need for mechanical ve

SUBMITTER: Serpa PH 

PROVIDER: S-EPMC9275031 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature