DUB1 suppresses Hippo signaling by modulating TAZ protein expression in gastric cancer.

ABSTRACT:

Background

The Hippo pathway functions as a tumor suppressor pathway in human cancers, while dysfunction of the Hippo pathway is frequently observed in malignancies. Although YAP/TAZ activity is tightly controlled by the phosphorylation cascade of the MST-LATS-YAP/TAZ axis, it is still unclear why the YAP/TAZ proteins are activated in human cancers despite Hippo pathway activation. Recent studies have suggested that in addition to phosphorylation, several other posttranslational modifications, including ubiquitination, also play critical roles in modulating TAZ function.

Methods

We used several gastric cancer cell lines and performed western blot analysis, real-time PCR, immunoprecipitation assays, and in vitro ubiquitination assays and established a xenograft mouse model.

SUBMITTER: Wang D 

PROVIDER: S-EPMC9275142 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature