Recombinant human klotho protects against hydrogen peroxide-mediated injury in human retinal pigment epithelial cells via the PI3K/Akt-Nrf2/HO-1 signaling pathway.

ABSTRACT: Globally, age-related macular degeneration (AMD) is a common irreversible ophthalmopathy. Oxidative stress of retinal pigment epithelial cells is involved in AMD occurrence and development. Klotho is an anti-aging protein with antioxidant properties. We investigated the protective properties of Klotho on hydrogen peroxide (H₂O₂)-induced injury of retinal pigment epithelial cells (ARPE-19 cells) and its associated pathomechanisms. We found that Klotho pretreatment for 24 h could up-regulate Bcl-2 levels, decrease the cleaved-caspase-3 and Bax levels, inhibit H₂O₂-induced ARPE-19 cell apoptosis, and promote cell proliferation. Klotho pretreatment inhibited the H₂O₂-mediated elevations of reactive oxygen species (ROS) in ARPE-1

SUBMITTER: Wen X 

PROVIDER: S-EPMC9275962 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature