Recombinant expression of hen egg white lysozyme with the assistance of xylanase fusion partner in Pichia pastoris.

ABSTRACT: Due to its bacteriolytic activity, hen egg white lysozyme (HEWL) is widely used in the feed, food, and pharmaceutical industries. However, its application is hindered by low protein expression levels in microbial expression systems. In this work, a novel fusion protein expression strategy was proposed for increasing the expression level of HEWL. First, HEWL, fused with a highly expressed fusion protein partner xylanase XynCDBFV, is expressed in Pichia pastoris. Secondly, a linker including endogenous protease cleavage sites was introduced between two fusion proteins in order to separate them directly during the secretion process. Finally, the results show that the supernatant of XynCDBFV-HEWL has a higher HEWL expression level and activity compared with HEWL only. It should be noted

SUBMITTER: Cui L 

PROVIDER: S-EPMC9275996 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature