A Fluorometric CYP19A1 (Aromatase) Activity Assay in Live Cells.

ABSTRACT: Inhibition of estrogen synthesis is an integral component of the frontline pharmacologic therapy for the treatment of estrogen receptor positive cancers. However, there is currently no direct, high-throughput-ready assay for aromatase (also known as CYP19A1) that can be performed in live cells. Herein we present a cell-based assay that allows for multiplexed assessment of enzyme activity, protein half-life, cell viability, and identification of inhibitors with slow off-rates.

SUBMITTER: Heidary DK 

PROVIDER: S-EPMC9276262 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature