PGC-1α induced mitochondrial biogenesis in stromal cells underpins mitochondrial transfer to melanoma.

ABSTRACT:

Introduction

Progress in the knowledge of metabolic interactions between cancer and its microenvironment is ongoing and may lead to novel therapeutic approaches. Until recently, melanoma was considered a glycolytic tumour due to mutations in mitochondrial-DNA, however, these malignant cells can regain OXPHOS capacity via the transfer of mitochondrial-DNA, a process that supports their proliferation in-vitro and in-vivo. Here we study how melanoma cells acquire mitochondria and how this process is facilitated from the tumour microenvironment.

Methods

Primary melanoma cells, and MSCs derived from patients were obtained. Genes' expression and DNA quantification was analysed using Real-time PCR. MSC migration, melanoma proliferation and tumour volume, in a xenograft subcutaneous

SUBMITTER: Kumar PR 

PROVIDER: S-EPMC9276678 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature