Fibroblast activation protein identifies Consensus Molecular Subtype 4 in colorectal cancer and allows its detection by ⁶⁸Ga-FAPI-PET imaging.

ABSTRACT:

Background

In colorectal cancer (CRC), the consensus molecular subtype 4 (CMS4) is associated with therapy resistance and poor prognosis. Clinical diagnosis of CMS4 is hampered by locoregional and temporal variables influencing CMS classification. Diagnostic tools that comprehensively detect CMS4 are therefore urgently needed.

Methods

To identify targets for molecular CMS4 imaging, RNA sequencing data of 3232 primary CRC patients were explored. Heterogeneity of marker expression in relation to CMS4 status was assessed by analysing 3-5 tumour regions and 91.103 single-tumour cells (7 and 29 tumours, respectively). Candidate marker expression was validated in CMS4 peritoneal metastases (PM; n = 59). Molecular imaging was performed using the ⁶⁸Ga-DOTA-FAPI-46 PET tra

SUBMITTER: Strating E 

PROVIDER: S-EPMC9276750 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature