Quantitative Analysis of the Protein Methylome Reveals PARP1 Methylation is involved in DNA Damage Response.

ABSTRACT: Protein methylation plays important roles in DNA damage response. To date, proteome-wide profiling of protein methylation upon DNA damage has been not reported yet. In this study, using HILIC affinity enrichment combined with MS analysis, we conducted a quantitative analysis of the methylated proteins in HEK293T cells in response to IR treatment. In total, 235 distinct methylation sites responding to IR treatment were identified, and 38% of them were previously unknown. Multiple RNA-binding proteins were differentially methylated upon DNA damage stress. Furthermore, we identified 14 novel methylation sites in DNA damage response-related proteins. Moreover, we validated the function of PARP1 K23 methylation in repairing IR-induced DNA lesions. K23 methylation deficiency sensitizes cancer ce

SUBMITTER: Wang X 

PROVIDER: S-EPMC9277342 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature