Dendritic cell-mediated cross presentation of tumor-derived peptides is biased against plasma membrane proteins.

ABSTRACT:

Background

For effective tumor elimination, cytotoxic CD8⁺ T cells must recognize tumor-derived antigens presented on class I major histocompatibility complex (MHC-I). Despite a general association between the expression of immunogenic antigens, typically neoantigens, and response to immunotherapy, the majority of patients lack strong endogenous responses to most putative neoantigens due to mechanisms that are not well understood. Cytotoxic CD8⁺ T-cell responses are induced by dendritic cells (DCs) cross-presenting tumor-derived peptides on MHC-I. We hypothesized that cross presentation may form an unappreciated source of bias in the induction of cytotoxic T-cell responses.

Methods

We used stable isotope labeling of amino acids combined with immunopepti

SUBMITTER: Fessenden TB 

PROVIDER: S-EPMC9277371 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature