Encapsulated droplet interface bilayers as a platform for high-throughput membrane studies.

ABSTRACT: Whilst it is highly desirable to produce artificial lipid bilayer arrays allowing for systematic high-content screening of membrane conditions, it remains a challenge due to the combined requirements of scaled membrane production, simple measurement access, and independent control over individual bilayer experimental conditions. Here, droplet bilayers encapsulated within a hydrogel shell are output individually into multi-well plates for simple, arrayed quantitative measurements. The afforded experimental throughput is used to conduct a 2D concentration screen characterising the synergistic pore-forming peptides Magainin2 and PGLa. Maximal enhanced activity is revealed at equimolar peptide concentrations via a membrane dye leakage assay, a finding consistent with models proposed fro

SUBMITTER: Baxani DK 

PROVIDER: S-EPMC9277618 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature