MYCN protein stability is a better prognostic indicator in neuroblastoma.

ABSTRACT:

Objective

MYCN oncogene amplification is associated with treatment failure and poor prognosis in neuroblastoma. To date, most detection methods of MYCN focus on DNA copy numbers instead of protein expression, which is the real one performing biological function, for poor antibodies. The current investigation was to explore a fast and reliable way to detect MYCN protein expression and evaluate its performance in predicting prognosis.

Methods

Several MYCN antibodies were used to detect MYCN protein expression by immunohistochemistry (IHC), and one was chosen for further study. We correlated the IHC results of MYCN from 53 patients with MYCN fluorescence in situ hybridization (FISH) and identified the sensitivity and specificity of IHC. The relationship between patient prognosi

SUBMITTER: Yang Y 

PROVIDER: S-EPMC9277955 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature