The dynamics of t1 adenosine binding on human Argonaute 2: Understanding recognition with conformational selection.

ABSTRACT: The control of expression in genetic regulation is a fundamental process for cell life. In RNA-mediated silencing, human Argonaute-2 protein (hAgo2) uses sequence information encoded in small RNAs (guide) to identify complementary sites in messenger RNAs (target) for repression. The specificity of this molecular recognition lies at the basis of the mechanisms that control the expression of thousands of genes, which necessarily requires a fine tuning of complex events. Among these, the binding of the first nucleotide of the target RNA (t1) is emerging as an important modulator of hAgo2-mediated machinery. Using atomistic molecular dynamics-derived analyses, we address the mechanism behind t1-dependent regulation and study the impact of different t1 nucleotides (t1A, t1C, t1G, t1U) on the co

SUBMITTER: Rinaldi S 

PROVIDER: S-EPMC9278005 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature