Simultaneous Raman and infrared spectroscopy: a novel combination for studying bacterial infections at the single cell level.

ABSTRACT: Sepsis is a life-threatening clinical condition responsible for approximately 11 million deaths worldwide. Rapid and accurate identification of pathogenic bacteria and its antimicrobial susceptibility play a critical role in reducing the morbidity and mortality rates related to sepsis. Raman and infrared spectroscopies have great potential to be used as diagnostic tools for rapid and culture-free detection of bacterial infections. Despite numerous reports using both methods to analyse bacterial samples, there is to date no study collecting both Raman and infrared signatures from clinical samples simultaneously due to instrument incompatibilities. Here, we report for the first time the use of an emerging technology that provides infrared signatures via optical photothermal infrared (

SUBMITTER: Lima C 

PROVIDER: S-EPMC9278432 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature