Activity of acute pancreatitis is modified by secreted protein acidic and rich in cysteine ablation.

ABSTRACT:

Background

Acute pancreatitis (AP) is a frequent cause for hospitalization. However, molecular determinants that modulate severity of experimental pancreatitis are only partially understood.

Objective

To investigate the role of secreted protein acidic and rich in cysteine (SPARC) during cerulein-induced AP in mice.

Methods

AP was induced by repeated cerulein injections in SPARC knock-out mice (SPARC^-/- ) and control littermates (SPARC^+/+ ). Secreted protein acidic and rich in cysteine expression and severity of AP were determined by histopathological scoring, immunohistochemistry, and biochemical assays. For functional analysis, primary murine acinar cell cultures with subsequent amylase release assays were employed. Proteome profiler assay and E

SUBMITTER: Ammer-Herrmenau C 

PROVIDER: S-EPMC9278565 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature