Combine Cryo-EM Density Map and Residue Contact for Protein Structure Prediction - A Case Study.

ABSTRACT: Cryo-electron microscopy is a major structure determination technique for large molecular machines and membrane-associated complexes. Although atomic structures have been determined directly from cryo-EM density maps with high resolutions, current structure determination methods for medium resolution (5 to 10 Å) cryo-EM maps are limited by the availability of structure templates. Secondary structure traces are lines detected from a cryo-EM density map for α-helices and β-strands of a protein. When combined with secondary structure sequence segments predicted from a protein sequence, it is possible to generate a set of likely topologies of α-traces and β-sheet traces. A topology describes the overall folding relationship among secondary structures; it is a critical piece of information for

SUBMITTER: Alshammari M 

PROVIDER: S-EPMC9279007 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature