A peroxisomal ubiquitin ligase complex forms a retrotranslocation channel.

ABSTRACT: Peroxisomes are ubiquitous organelles that house various metabolic reactions and are essential for human health^1-4. Luminal peroxisomal proteins are imported from the cytosol by mobile receptors, which then recycle back to the cytosol by a poorly understood process^1-4. Recycling requires receptor modification by a membrane-embedded ubiquitin ligase complex comprising three RING finger domain-containing proteins (Pex2, Pex10 and Pex12)^5,6. Here we report a cryo-electron microscopy structure of the ligase complex, which together with biochemical and in vivo experiments reveals its function as a retrotranslocation channel for peroxisomal import receptors. Each subunit of the complex contributes five transmembrane segments that co-assemble into an open channel.

SUBMITTER: Feng P 

PROVIDER: S-EPMC9279156 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature