An acoustofluidic scanning nanoscope using enhanced image stacking and processing.

ABSTRACT: Nanoscale optical resolution with a large field of view is a critical feature for many research and industry areas, such as semiconductor fabrication, biomedical imaging, and nanoscale material identification. Several scanning microscopes have been developed to resolve the inverse relationship between the resolution and field of view; however, those scanning microscopes still rely upon fluorescence labeling and complex optical systems. To overcome these limitations, we developed a dual-camera acoustofluidic nanoscope with a seamless image merging algorithm (alpha-blending process). This design allows us to precisely image both the sample and the microspheres simultaneously and accurately track the particle path and location. Therefore, the number of images required to capture the entire fi

SUBMITTER: Jin G 

PROVIDER: S-EPMC9279327 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature