Loop-extrusion and polymer phase-separation can co-exist at the single-molecule level to shape chromatin folding.

ABSTRACT: Loop-extrusion and phase-separation have been proposed as mechanisms that shape chromosome spatial organization. It is unclear, however, how they perform relative to each other in explaining chromatin architecture data and whether they compete or co-exist at the single-molecule level. Here, we compare models of polymer physics based on loop-extrusion and phase-separation, as well as models where both mechanisms act simultaneously in a single molecule, against multiplexed FISH data available in human loci in IMR90 and HCT116 cells. We find that the different models recapitulate bulk Hi-C and average multiplexed microscopy data. Single-molecule chromatin conformations are also well captured, especially by phase-separation based models that better reflect the experimentally reported segregati

SUBMITTER: Conte M 

PROVIDER: S-EPMC9279381 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature