CRISPR Del/Rei: a simple, flexible, and efficient pipeline for scarless genome editing.
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ABSTRACT:
SUBMITTER: Feuer KL
PROVIDER: S-EPMC9279498 | biostudies-literature | 2022 Jul
REPOSITORIES: biostudies-literature
Publications
CRISPR Del/Rei: a simple, flexible, and efficient pipeline for scarless genome editing.
Feuer Kyra L KL Wahbeh Marah H MH Yovo Christian C Rabie Eman E Lam Anh-Thu N AN Abdollahi Sara S Young Lindsay J LJ Rike Bailey B Umamageswaran Akul A Avramopoulos Dimitrios D
Scientific reports 20220713 1
Scarless genome editing of induced pluripotent stem cells (iPSCs) is crucial for the precise modeling of genetic disease. Here we present CRISPR Del/Rei, a two-step deletion-reinsertion strategy with high editing efficiency and simple PCR-based screening that generates isogenic clones in ~ 2 months. We apply our strategy to edit iPSCs at 3 loci with only rare off target editing. ...[more]
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