CRISPR Del/Rei: a simple, flexible, and efficient pipeline for scarless genome editing.

ABSTRACT: Scarless genome editing of induced pluripotent stem cells (iPSCs) is crucial for the precise modeling of genetic disease. Here we present CRISPR Del/Rei, a two-step deletion-reinsertion strategy with high editing efficiency and simple PCR-based screening that generates isogenic clones in ~ 2 months. We apply our strategy to edit iPSCs at 3 loci with only rare off target editing.

SUBMITTER: Feuer KL 

PROVIDER: S-EPMC9279498 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature