Improvement of surgical techniques for orthotopic single lung transplantation in rats.

ABSTRACT:

Background

Although orthotopic single lung transplantation in rats has long been established, this model is still highly challenging. Therefore, we made several modifications in anesthesia, lung extraction, vascular clamp, and transplantation procedures for this model.

Methods

Fifty cases of rat left lung transplantation were performed using traditional procedures and modified surgical techniques, respectively. Two hundred Sprague Dawley male rats, half as donors and half as recipients, were randomized equally to the two groups. The modifications included orotracheal intubation via a video laryngoscope, retrograde perfusion following anterograde perfusion, a Rummel tourniquet for the occlusion of pulmonary vessels, flushing the vessels and cuffs before anastomosis with hepar

SUBMITTER: Gao P 

PROVIDER: S-EPMC9279794 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature