Integrated Stress Response Regulation of Corneal Epithelial Cell Motility and Cytokine Production.

ABSTRACT:

Purpose

To investigate the effect of an active integrated stress response (ISR) on human corneal epithelial cell motility and cytokine production.

Methods

ISR agonists tunicamycin (TUN) and SAL003 (SAL) were used to stimulate the ISR in immortalized corneal epithelial cell lines, primary human limbal epithelial stem cells, and ex vivo human corneas. Reporter lines for ISR-associated transcription factors activating transcription factor 4 (ATF4) and XBP1 activity were generated to visualize pathway activity in response to kinase-specific agonists. Scratch assays and multiplex magnetic bead arrays were used to investigate the effects of an active ISR on scratch wounds and cytokine production. A C/EBP homologous protein (CHOP) knockout cell line was generated to investigate the

SUBMITTER: Chu HS 

PROVIDER: S-EPMC9279922 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature