MiR-548d-3p inhibits the invasion and migration of gastric cancer cells by targeting GKN1.

ABSTRACT:

Background

The aim of this study was to explore the function and mechanism of GKN1 in gastric cancer (GC) progression.

Methods

Firstly, we used GEO2R to perform differential gene analysis on GSE26942 and GSE79973 and constructed the protein-protein interaction network of differential genes by STRING. Next, the cytoHubba, Mcode plugins, and GEPIA were used to obtain our follow-up research object GKN1. Then, the function of GKN1 in GC was verified by scratch and transwell assay in GC cells. We further analyzed the genes related to GKN1 through LinkedOmics, and exported top 100 genes positively or negatively correlated with GKN1. Meanwhile, Metascape was performed on these genes. Finally, we analyzed the miRNAs that bind to GKN1 through the miRDB and verified the correlation be

SUBMITTER: Yu S 

PROVIDER: S-EPMC9279950 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature