Optimal whole blood dilution protocol for preprocessing samples prior to circulating tumor cell capture by size-based isolation.

ABSTRACT:

Background

This study compared whole blood dilution versus density gradient centrifugation for pre-processing blood samples prior to circulating tumor cell (CTC) capture on the efficiency of CTC separation by size-based isolation.

Materials and methods

Whole blood from a healthy volunteer spiked with SKBR3 cells was used to optimize the whole blood dilution protocol for sample volume, dilution ratio, and paraformaldehyde (PFA) concentration. Whole blood from healthy volunteers spiked with SKBR3, A549, or PC3 cells, and whole blood from patients with advanced gastric, esophageal, or liver cancer, was used to compare pre-processing by the optimal whole blood dilution protocol with density-gradient centrifugation. All statistical evaluations were performed using Student t test

SUBMITTER: Fu T 

PROVIDER: S-EPMC9279958 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature