Measurement comparability of insulin assays using conventional immunoassay kits.

ABSTRACT:

Background

The standardization of measurement aims to achieve comparability of results regardless of the analytical methods and the laboratory where analyses are carried out. In this paper, a comparison of results from several immunoassay-based insulin analysis kits is described, and the steps necessary to improve comparability are discussed.

Methods

Four manual enzyme-linked immunosorbent assay (ELISA) kits produced by Mercodia, Alpco, Epitope Diagnostics, and Abcam, and three automated chemiluminescent (CLIA) insulin assay kits (Siemens Centaur XP, Unicel Dxl800, Cobas e801) were compared by analyzing human serum samples and certified reference materials for human insulin.

Results

The seven evaluated assay kits showed substantial discrepancies in the results, with r

SUBMITTER: Rosli N 

PROVIDER: S-EPMC9279959 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature